In this WiKi we provide examples on how to analyze and process FCS and imaging data generated on Zeiss LSM microscopes using [microscopy pipeline constructor](https://git.embl.de/politi/mypic) or
[FCSRunner](https://git.embl.de/politi/fcsrunner). The processed data can be used to generate a calibration curve for converting pixel fluorescence intensities to concentrations.
The order
The order of the processing step is
[**1. Generate bleach corrected correlation functions using Fluctuation Analyzer 4G**](./FA_Load_and_Correlate)
